LOCUS       40924_8006       11427 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCAG-dCas9TET1CD DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Morita S, Noguchi H, Horii T, Nakabayashi K, Kimura M, Okamura K, 
            Sakai A, Nakashima H, Hata K, Nakashima K, Hatada I.
  TITLE     Targeted DNA demethylation in vivo using dCas9-peptide repeat and 
            scFv-TET1 catalytic domain fusions
  JOURNAL   Nat. Biotechnol. 34 (10), 1060-1065 (2016)
  PUBMED    27571369
REFERENCE   2  (bases 1 to 11427)
  AUTHORS   Hatada I, Morita S.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2016) Contact:Izuho Hatada Gunma University, 
            Institute for Molecular and Cellular Regulation; 3-39-15 
            Showa-machi, Maebashi, Gunma 371-8512, Japan URL 
            :http://epigenome.dept.showa.gunma-u.ac.jp/~hatada/
REFERENCE   3  (bases 1 to 11427)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11427)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2016"; volume: "34"; issue: "10"; pages: 
            "1060-1065"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JUL-2016) Contact:Izuho Hatada Gunma University, Institute for 
            Molecular and Cellular Regulation"; volume: " 3-39-15 Showa-machi, 
            Maebashi, Gunma 371-8512, Japan URL :http"; pages: 
            "//epigenome.dept.showa.gunma-u.ac.jp/~hatada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        367..642
                     /label=chicken beta-actin promoter
     intron          643..1651
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1725..1751
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1755..1775
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1785..5885
                     /codon_start=1
                     /label=dCas9
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5889..5909
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8215..8336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(8343..8798)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8825..8929
                     /label=AmpR promoter
     promoter        8931..9288
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             9323..10114
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    10349..10396
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10725..11313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      421 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      481 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
      541 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      601 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgttgcct
      661 tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      721 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      781 gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa gccttaaagg gctccgggag
      841 ggccctttgt gcggggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
      901 cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      961 tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     1021 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     1081 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
     1141 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
     1201 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     1261 gctcggggga ggggcgcggc ggccccggag cgccggcggc tgtcgaggcg cggcgagccg
     1321 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1381 ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cgggcgaagc
     1441 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     1501 gtccccttct ccatctccag cctcggggct gccgcagggg gacggctgcc ttcggggggg
     1561 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     1621 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttgttgtgct
     1681 gtctcatcat tttggcaaag aattctgcag tcgacggtac catgtaccca tacgatgttc
     1741 cagattacgc ttcgccgaag aaaaagcgca aggtcgaagc gtccgacaag aagtacagca
     1801 tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
     1861 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
     1921 tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg ctgaagagaa
     1981 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
     2041 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
     2101 tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg
     2161 cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg gacagcaccg
     2221 acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag ttccggggcc
     2281 acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag ctgttcatcc
     2341 agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg
     2401 acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa aatctgatcg
     2461 cccagctgcc cggcgagaag aagaatggcc tgttcggcaa cctgattgcc ctgagcctgg
     2521 gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa ctgcagctga
     2581 gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc gaccagtacg
     2641 ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc gacatcctga
     2701 gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag agatacgacg
     2761 agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt
     2821 acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt gacggcggag
     2881 ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg gacggcaccg
     2941 aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg accttcgaca
     3001 acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg cggcggcagg
     3061 aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc ctgaccttcc
     3121 gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc tggatgacca
     3181 gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac aagggcgctt
     3241 ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc aacgagaagg
     3301 tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag ctgaccaaag
     3361 tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag cagaaaaagg
     3421 ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg
     3481 actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt
     3541 tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac aaggacttcc
     3601 tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg acactgtttg
     3661 aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc gacgacaaag
     3721 tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc cggaagctga
     3781 tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg aagtccgacg
     3841 gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc tttaaagagg
     3901 acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac attgccaatc
     3961 tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg gtggacgagc
     4021 tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg gccagagaga
     4081 accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg atcgaagagg
     4141 gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac acccagctgc
     4201 agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac gtggaccagg
     4261 aactggacat caaccggctg tccgactacg atgtggacgc catcgtgcct cagagctttc
     4321 tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac cggggcaaga
     4381 gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg cggcagctgc
     4441 tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc gagagaggcg
     4501 gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa acccggcaga
     4561 tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac gacgagaatg
     4621 acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg tccgatttcc
     4681 ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg
     4741 cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg
     4801 agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc
     4861 aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg aactttttca
     4921 agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc gagacaaacg
     4981 gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg cggaaagtgc
     5041 tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca
     5101 aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag aaggactggg
     5161 accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg
     5221 ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg ctggggatca
     5281 ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa gccaagggct
     5341 acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg ttcgagctgg
     5401 aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga aacgaactgg
     5461 ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag aagctgaagg
     5521 gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag cactacctgg
     5581 acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc gacgctaatc
     5641 tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga gagcaggccg
     5701 agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc ttcaagtact
     5761 ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg gacgccaccc
     5821 tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct cagctgggag
     5881 gcgacagccc caagaagaag agaaaggtgg aggccagcgg tggcggagga tccgaactgc
     5941 ccacctgcag ctgtcttgat cgagttatac aaaaagacaa aggcccatat tatacacacc
     6001 ttggggcagg accaagtgtt gctgctgtca gggaaatcat ggagaatagg tatggtcaaa
     6061 aaggaaacgc aataaggata gaaatagtag tgtacaccgg taaagaaggg aaaagctctc
     6121 atgggtgtcc aattgctaag tgggttttaa gaagaagcag tgatgaagaa aaagttcttt
     6181 gtttggtccg gcagcgtaca ggccaccact gtccaactgc tgtgatggtg gtgctcatca
     6241 tggtgtggga tggcatccct cttccaatgg ccgaccggct atacacagag ctcacagaga
     6301 atctaaagtc atacaatggg caccctaccg acagaagatg caccctcaat gaaaatcgta
     6361 cctgtacatg tcaaggaatt gatccagaga cttgtggagc ttcattctct tttggctgtt
     6421 catggagtat gtactttaat ggctgtaagt ttggtagaag cccaagcccc agaagattta
     6481 gaattgatcc aagctctccc ttacatgaaa aaaaccttga agataactta cagagtttgg
     6541 ctacacgatt agctccaatt tataagcagt atgctccagt agcttaccaa aatcaggtgg
     6601 aatatgaaaa tgttgcccga gaatgtcggc ttggcagcaa ggaaggtcgt cccttctctg
     6661 gggtcactgc ttgcctggac ttctgtgctc atccccacag ggacattcac aacatgaata
     6721 atggaagcac tgtggtttgt accttaactc gagaagataa ccgctctttg ggtgttattc
     6781 ctcaagatga gcagctccat gtgctacctc tttataagct ttcagacaca gatgagtttg
     6841 gctccaagga aggaatggaa gccaagatca aatctggggc catcgaggtc ctggcacccc
     6901 gccgcaaaaa aagaacgtgt ttcactcagc ctgttccccg ttctggaaag aagagggctg
     6961 cgatgatgac agaggttctt gcacataaga taagggcagt ggaaaagaaa cctattcccc
     7021 gaatcaagcg gaagaataac tcaacaacaa caaacaacag taagccttcg tcactgccaa
     7081 ccttagggag taacactgag accgtgcaac ctgaagtaaa aagtgaaacc gaaccccatt
     7141 ttatcttaaa aagttcagac aacactaaaa cttattcgct gatgccatcc gctcctcacc
     7201 cagtgaaaga ggcatctcca ggcttctcct ggtccccgaa gactgcttca gccacaccag
     7261 ctccactgaa gaatgacgca acagcctcat gcgggttttc agaaagaagc agcactcccc
     7321 actgtacgat gccttcggga agactcagtg gtgccaatgc agctgctgct gatggccctg
     7381 gcatttcaca gcttggcgaa gtggctcctc tccccaccct gtctgctcct gtgatggagc
     7441 ccctcattaa ttctgagcct tccactggtg tgactgagcc gctaacgcct catcagccaa
     7501 accaccagcc ctccttcctc acctctcctc aagaccttgc ctcttctcca atggaagaag
     7561 atgagcagca ttctgaagca gatgagcctc catcagacga acccctatct gatgaccccc
     7621 tgtcacctgc tgaggagaaa ttgccccaca ttgatgagta ttggtcagac agtgagcaca
     7681 tctttttgga tgcaaatatt ggtggggtgg ccatcgcacc tgctcacggc tcggttttga
     7741 ttgagtgtgc ccggcgagag ctgcacgcta ccactcctgt tgagcacccc aaccgtaatc
     7801 atccaacccg cctctccctt gtcttttacc agcacaaaaa cctaaataag ccccaacatg
     7861 gttttgaact aaacaagatt aagtttgagg ctaaagaagc taagaataag aaaatgaagg
     7921 cctcagagca aaaagaccag gcagctaatg aaggtccaga acagtcctct gaagtaaatg
     7981 aattgaacca aattccttct cataaagcat taacattaac ccatgacaat gttgtcaccg
     8041 tgtcccctta tgctctcaca cacgttgcgg ggccctataa ccattgggtc tgagcggccg
     8101 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     8161 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     8221 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8281 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     8341 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     8401 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     8461 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     8521 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     8581 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     8641 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     8701 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     8761 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     8821 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8881 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     8941 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9001 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9061 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9121 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9181 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9241 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     9301 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     9361 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     9421 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     9481 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     9541 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     9601 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     9661 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     9721 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     9781 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     9841 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     9901 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     9961 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
    10021 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
    10081 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
    10141 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
    10201 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    10261 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
    10321 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
    10381 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
    10441 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
    10501 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
    10561 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    10621 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10681 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10741 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10801 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10861 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10921 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10981 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    11041 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    11101 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    11161 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    11221 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11281 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11341 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    11401 gtggataacc gtattaccgc catgcat
//
